SeedGenes Profile: TTN 5

Gene Information Gene Symbol TTN 5 Chromosome Locus At2g18390   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol HAL (G. Jurgens) Sequence Identity Gene Class Embryo Defective Predicted Function ARL2 GTPase Function Details Postulated to interact with TTN1 protein to regulate b-tubulin folding and microtubule dynamics Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Sequenced Mutant Allele Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 1361 nucleotides mRNA: 879 nucleotides Protein:   185 amino acids cDNA Status FLcDNA Available: AY088331 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (98%) Model Genomes:   Danio rerio (67%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 265337_at

Mutant Information Allele Symbol ttn 5-2 Mutant Class Embryo Defective Source of Mutant W. Lukowitz Mutant Line Number 19-22 Ecotype Landsberg erecta Genetic Background   ABRC Stock   Mutagen Treatment EMS Insertion Mutant No Flanking Sequence   Location of Mutation Exon 3 Terminal Phenotype Preglobular  Details Nomarski Phenotype Preglobular  Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 28.4 % Chi - Square 4.0 Seeds Counted 670 Percent Top Half 51.6 % Chi - Square 0.2 Average Seed Length 460 ± 40 µm Average Embryo Length 15 ± 0 µm