SeedGenes Profile: TTN 5

Gene Information Gene Symbol TTN 5 Chromosome Locus At2g18390   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol HAL (G. Jurgens) Sequence Identity Gene Class Embryo Defective Predicted Function ARL2 GTPase Function Details Postulated to interact with TTN1 protein to regulate b-tubulin folding and microtubule dynamics Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Sequenced Mutant Allele Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 1361 nucleotides mRNA: 879 nucleotides Protein:   185 amino acids cDNA Status FLcDNA Available: AY088331 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (98%) Model Genomes:   Danio rerio (67%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 265337_at

Mutant Information Allele Symbol ttn 5-1 ttn 5-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta W. Lukowitz Mutant Line Number 18048 19-22 Ecotype Uncertain Landsberg erecta Genetic Background     ABRC Stock CS16077   Mutagen Treatment T-DNA EMS Insertion Mutant Yes   Details No Flanking Sequence     Location of Mutation Intron 3 to Exon 4 Exon 3 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Preglobular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.3 % 28.4 % Chi - Square 0.2 4.0 Seeds Counted 942 670 Percent Top Half 51.5 % 51.6 % Chi - Square 0.2 0.2 Average Seed Length 430 ± 40 µm 460 ± 40 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm