SeedGenes Profile: TWN 3

Gene Information Gene Symbol TWN 3 Chromosome Locus At1g74970   Alleles in Database 1 Identity Confidence Not Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Ribosomal Protein S9 Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2004 Predicted Sequences Gene: 1773 nucleotides mRNA: 958 nucleotides Protein:   208 amino acids cDNA Status FLcDNA Available: AK176773 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (91%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 262172_at

Mutant Information Allele Symbol twn 3 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 75709 Ecotype Columbia Genetic Background qrt ABRC Stock CS24062 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Intron 1 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2004 Seed Color Pale Yellow Embryo Color Pale Yellow Percent Mutant Seeds 20.6 % Chi - Square 10.5 Seeds Counted 1028 Percent Top Half 49.5 % Chi - Square <0.1 Average Seed Length 430 ± 60 µm Average Embryo Length 230 ± 130 µm