OSU Syngenta vbi

SeedGenes Profile: EMB 1273

Gene Information
Gene Symbol EMB 1273
Chromosome Locus At1g49510   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Unknown
Function Details None
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:1722 nucleotides
mRNA: 1196 nucleotides
Protein:  240 amino acids
cDNA Status FLcDNA Available: AK227372
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (78%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 262394_at
    
Mutant Information
Allele Symbol emb 1273-1emb 1273-2
Mutant Class Embryo DefectiveSeed Pigment
Source of Mutant Meinke / SyngentaMeinke / Salk
Mutant Line Number 16479SALK_004621
Ecotype UncertainColumbia
Genetic Background   
ABRC Stock CS16016CS24063
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Intron 1 to Exon 3Exon 1
Terminal Phenotype Cotyledon  DetailsPigment Defective  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002March, 2004
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 26.9 %25.4 %
Chi - Square 2.00.1
Seeds Counted 1010913
Percent Top Half 47.8 %48.7 %
Chi - Square 0.50.1
Average Seed Length 480 ± 40 µm550 ± 60 µm
Average Embryo Length 330 ± 50 µm460 ± 50 µm