OSU Syngenta vbi

SeedGenes Profile: EMB 1417

Gene Information
Gene Symbol EMB 1417
Chromosome Locus At4g21190   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function 5' Adenylsulfate Reductase (APR3)
Function Details Sulfate reduction
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / SAIL (1 allele)
Database Release September, 2002
Predicted Sequences
Gene:1818 nucleotides
mRNA: 1308 nucleotides
Protein:  307 amino acids
cDNA Status FLcDNA Available: AK176577
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Cotton (Gossypium) (72%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 254427_at
    
Mutant Information
Allele Symbol emb 1417-1emb 1417-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SAIL
Mutant Line Number 21455SAIL_110_B03
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16120 
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Upstream to Exon 1 in 5' UTR3' UTR
Terminal Phenotype Cotyledon  DetailsGlobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release September, 2002December, 2010
Seed Color White 
Embryo Color White 
Percent Mutant Seeds 23.4 %25.7 %
Chi - Square 2.20.2
Seeds Counted 1098509
Percent Top Half 52.9 % 
Chi - Square 0.9 
Average Seed Length 500 ± 60 µm 
Average Embryo Length 180 ± 100 µm