OSU Syngenta vbi

SeedGenes Profile: EMB 1579

Gene Information
Gene Symbol EMB 1579
Chromosome Locus At2g03150   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details Putative calcium binding protein
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:7233 nucleotides
mRNA: 4375 nucleotides
Protein:  1340 amino acids
cDNA Status FLcDNA Available: AK228824
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Soybean (Glycine max) (61%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
mRNA Expression Data NASCArray Spot History for 266727_at
Mutant Information
Allele Symbol emb 1579-1emb 1579-2emb 1579-3
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SyngentaMeinke / Salk
Mutant Line Number 2964957679SALK_007142
Ecotype ColumbiaColumbiaColumbia
Genetic Background    
ABRC Stock CS16026CS16027CS24068
Mutagen Treatment T-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)Details 
Location of Mutation Intron 18Exon 6 to Exon 12Exon 3
Terminal Phenotype Cotyledon  DetailsCotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release March, 2002March, 2002March, 2004
Seed Color WhiteWhite to Pale YellowPale Yellow
Embryo Color WhiteWhite to Pale YellowPale Yellow
Percent Mutant Seeds 21.9 %25.3 %23.0 %
Chi - Square 4.9<0.12.7
Seeds Counted 96311151202
Percent Top Half 55.5 %56.0 %53.3 %
Chi - Square
Average Seed Length 440 ± 60 µm450 ± 30 µm500 ± 50 µm
Average Embryo Length 120 ± 110 µm240 ± 120 µm350 ± 90 µm