SeedGenes Profile: EMB 1579

Gene Information Gene Symbol EMB 1579 Chromosome Locus At2g03150   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Putative calcium binding protein Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 7233 nucleotides mRNA: 4375 nucleotides Protein:   1340 amino acids cDNA Status FLcDNA Available: AK228824 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Soybean (Glycine max) (61%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 266727_at

Mutant Information Allele Symbol emb 1579-1 emb 1579-2 emb 1579-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Salk Mutant Line Number 29649 57679 SALK_007142 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock CS16026 CS16027 CS24068 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details   Location of Mutation Intron 18 Exon 6 to Exon 12 Exon 3 Terminal Phenotype Cotyledon  Details Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 March, 2004 Seed Color White White to Pale Yellow Pale Yellow Embryo Color White White to Pale Yellow Pale Yellow Percent Mutant Seeds 21.9 % 25.3 % 23.0 % Chi - Square 4.9 <0.1 2.7 Seeds Counted 963 1115 1202 Percent Top Half 55.5 % 56.0 % 53.3 % Chi - Square 2.5 2.1 1.2 Average Seed Length 440 ± 60 µm 450 ± 30 µm 500 ± 50 µm Average Embryo Length 120 ± 110 µm 240 ± 120 µm 350 ± 90 µm