SeedGenes Profile: EMB 1705

Gene Information Gene Symbol EMB 1705 Chromosome Locus At5g22370   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol QQT 1 (M. Devic) Sequence Identity QQT 1 (M. Devic) Complemetation Test Gene Class Embryo Defective Predicted Function GPN-Loop GTPase 2 Function Details Microtubule organization Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Border Recovery Devic / Versailles (1 allele) Database Release September, 2002 Predicted Sequences Gene: 2606 nucleotides mRNA: 1195 nucleotides Protein:   298 amino acids cDNA Status FLcDNA Available: AK221541 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (85%) Model Genomes:   Danio rerio (59%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 249931_at

Mutant Information Allele Symbol emb 1705-1 emb 1705-2 qqt 1 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Devic / Versailles Mutant Line Number 35154 SALK_059852 EIC6 Ecotype Columbia Columbia Wassilewskija Genetic Background       ABRC Stock CS16132 CS24073   Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Intron 2 Intron 2 Intron 2 Terminal Phenotype Transition  Details Preglobular  Details Preglobular Nomarski Phenotype   Images   Images Preglobular Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release September, 2002 March, 2004 October, 2007 Seed Color Pale Yellow-Green White   Embryo Color Pale Yellow-Green White   Percent Mutant Seeds 26.2 % 22.9 %   Chi - Square 0.8 1.3   Seeds Counted 1059 622   Percent Top Half 49.8 % 48.3 %   Chi - Square <0.1 0.2   Average Seed Length 510 ± 50 µm 310 ± 70 µm   Average Embryo Length 40 ± 60 µm 15 ± 0 µm