SeedGenes Profile: EMB 1860

Gene Information Gene Symbol EMB 1860 Chromosome Locus At1g55900   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Inner Mitochondrial Membrane Protein (Tim50) Function Details Import of mitochondrial matrix proteins Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2661 nucleotides mRNA: 1534 nucleotides Protein:   376 amino acids cDNA Status FLcDNA Available: AY072140 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (75%) Model Genomes:   Saccharomyces cerevisiae (46%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 260596_at

Mutant Information Allele Symbol emb 1860-1 emb 1860-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 42230 SALK_116273 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16035   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 9 to 3' UTR Exon 5 Terminal Phenotype Preglobular  Details Not Available Nomarski Phenotype Globular  Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2004 Seed Color White   Embryo Color White   Percent Mutant Seeds 25.4 %   Chi - Square 0.1   Seeds Counted 1063   Percent Top Half 53.0 %   Chi - Square 1.0   Average Seed Length 410 ± 30 µm   Average Embryo Length 15 ± 0 µm