OSU Syngenta vbi

SeedGenes Profile: EMB 1865

Gene Information
Gene Symbol EMB 1865
Chromosome Locus At3g18390   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Chloroplast Splicing Factor (CFM1)
Function Details Splicing of group II introns in chloroplasts
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Myouga / Riken (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:4487 nucleotides
mRNA: 3021 nucleotides
Protein:  848 amino acids
cDNA Status FLcDNA Available: AY063908
Top BLAST Hits
Arabidopsis: AT3G01370.1 (43%)
Plant ESTs: Vitis vinifera (66%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 257717_at
    
Mutant Information
Allele Symbol emb 1865-1emb 1865-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMyouga / Riken
Mutant Line Number 42644RATM16-1117-1
Ecotype ColumbiaNossen
Genetic Background   
ABRC Stock CS16036 
Mutagen Treatment T-DNATransposon
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 2 
Terminal Phenotype Globular  DetailsGlobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002December, 2010
Seed Color White 
Embryo Color White 
Percent Mutant Seeds 30.3 %24.7 %
Chi - Square 9.1<0.1
Seeds Counted 604279
Percent Top Half 48.1 % 
Chi - Square 0.3 
Average Seed Length 510 ± 50 µm 
Average Embryo Length 70 ± 30 µm