OSU Syngenta vbi

SeedGenes Profile: EMB 1968

Gene Information
Gene Symbol EMB 1968
Chromosome Locus At1g21690   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Replication Factor C
Function Details ATPase required for DNA polymerase loading
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Salk (1 allele)
Border Recovery
Meinke / Riken (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:2984 nucleotides
mRNA: 1316 nucleotides
Protein:  339 amino acids
cDNA Status FLcDNA Available: AY056210
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (93%)
Model Genomes:  Homo sapiens (56%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 262501_at
    
Mutant Information
Allele Symbol emb 1968-1emb 1968-2emb 1968-3
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SalkMeinke / Riken
Mutant Line Number 51792SALK_049715RATM15-0487-1
Ecotype ColumbiaColumbiaNossen
Genetic Background    
ABRC Stock CS16161  
Mutagen Treatment T-DNAT-DNATransposon
Insertion Mutant Yes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)  
Location of Mutation Intron 3Intron 2Exon 2
Terminal Phenotype Preglobular  DetailsPreglobularPreglobular
Nomarski Phenotype Preglobular
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release March, 2003December, 2010December, 2010
Seed Color White  
Embryo Color White  
Percent Mutant Seeds 27.0 %21.9 %25.4 %
Chi - Square 2.11.8<0.1
Seeds Counted 970302248
Percent Top Half 53.1 %  
Chi - Square 1.0  
Average Seed Length 440 ± 40 µm  
Average Embryo Length 15 ± 0 µm