SeedGenes Profile: EMB 1968

Gene Information Gene Symbol EMB 1968 Chromosome Locus At1g21690   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Replication Factor C Function Details ATPase required for DNA polymerase loading Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Salk (1 allele) Border Recovery Meinke / Riken (1 allele) Database Release March, 2003 Predicted Sequences Gene: 2984 nucleotides mRNA: 1316 nucleotides Protein:   339 amino acids cDNA Status FLcDNA Available: AY056210 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (93%) Model Genomes:   Homo sapiens (56%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 262501_at

Mutant Information Allele Symbol emb 1968-1 emb 1968-2 emb 1968-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Meinke / Riken Mutant Line Number 51792 SALK_049715 RATM15-0487-1 Ecotype Columbia Columbia Nossen Genetic Background       ABRC Stock CS16161     Mutagen Treatment T-DNA T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Intron 3 Intron 2 Exon 2 Terminal Phenotype Preglobular  Details Preglobular Preglobular Nomarski Phenotype Preglobular Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2003 December, 2010 December, 2010 Seed Color White     Embryo Color White     Percent Mutant Seeds 27.0 % 21.9 % 25.4 % Chi - Square 2.1 1.8 <0.1 Seeds Counted 970 302 248 Percent Top Half 53.1 %     Chi - Square 1.0     Average Seed Length 440 ± 40 µm     Average Embryo Length 15 ± 0 µm