SeedGenes Profile: EMB 1974

Gene Information Gene Symbol EMB 1974 Chromosome Locus At3g07060   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details None Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release September, 2002 Predicted Sequences Gene: 4484 nucleotides mRNA: 2412 nucleotides Protein:   693 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Medicago truncatula (39%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 258546_at

Mutant Information Allele Symbol emb 1974-1 emb 1974-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 46250 SALK_042472 Ecotype Columbia Columbia Genetic Background     ABRC Stock CS16112 CS24079 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 9 Intron 4 Terminal Phenotype Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2002 March, 2004 Seed Color White to Pale Yellow Pale Yellow Embryo Color White to Pale Yellow Pale Yellow Percent Mutant Seeds 17.3 % 24.1 % Chi - Square 24.6 0.4 Seeds Counted 775 988 Percent Top Half 44.8 % 51.7 % Chi - Square 1.5 0.2 Average Seed Length 450 ± 40 µm 490 ± 60 µm Average Embryo Length 160 ± 90 µm 320 ± 70 µm