SeedGenes Profile: EMB 1995

Gene Information Gene Symbol EMB 1995 Chromosome Locus At4g30580   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol LPAAT 1 (A. Huang) Sequence Identity ATS 2 (C. Benning) Sequence Identity Gene Class Embryo Defective Predicted Function Lysophosphatidic Acid Acyltransferase (LPAAT) Function Details Fatty acid and phospholipid metabolism Gene Evidence Border Recovery Benning / Salk (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Molecular Complementation Huang; Benning / Salk (1 allele) Border Recovery Huang; Benning / Salk (1 allele) Database Release March, 2004 Predicted Sequences Gene: 2195 nucleotides mRNA: 1291 nucleotides Protein:   356 amino acids cDNA Status FLcDNA Available: AK118028 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (81%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 253624_at

Mutant Information Allele Symbol emb 1995-1 emb 1995-2 ats 2-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 49488 SALK_073445 SALK_108812 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock CS24048     Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Exon 4 to Intron 4 Exon 4 Exon 5 Terminal Phenotype Cotyledon  Details Cotyledon Cotyledon Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Sequence Identity Database Release March, 2004 March, 2004 September, 2004 Seed Color White     Embryo Color White     Percent Mutant Seeds 28.6 %     Chi - Square 6.8     Seeds Counted 1012     Percent Top Half 44.6 %     Chi - Square 3.3     Average Seed Length 500 ± 70 µm     Average Embryo Length 210 ± 90 µm