OSU Syngenta vbi

SeedGenes Profile: EMB 2001

Gene Information
Gene Symbol EMB 2001
Chromosome Locus At2g22870   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details Putative ribosome biogenesis GTPase
Gene Evidence
Border Recovery
Meinke / Syngenta (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Database Release September, 2004
Predicted Sequences
Gene:1648 nucleotides
mRNA: 1105 nucleotides
Protein:  300 amino acids
cDNA Status FLcDNA Available: AY087277
Top BLAST Hits
Arabidopsis: AT5G11480.1 (54%)
Plant ESTs: Brassica napus (85%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 266801_at
    
Mutant Information
Allele Symbol emb 2001-1emb 2001-2
Mutant Class Embryo DefectiveSeed Pigment
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 5484698507
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock   
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence  Details  (Help)
Location of Mutation  Exon 1
Terminal Phenotype Transition  DetailsCotyledon
Nomarski Phenotype   Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release September, 2004September, 2004
Seed Color WhitePale Yellow
Embryo Color WhitePale Yellow
Percent Mutant Seeds 25.3 %24.9 %
Chi - Square 0.1<0.1
Seeds Counted 1105936
Percent Top Half 51.8 %48.5 %
Chi - Square 0.40.2
Average Seed Length 510 ± 60 µm 
Average Embryo Length 100 ± 40 µm