SeedGenes Profile: EMB 2036

Gene Information Gene Symbol EMB 2036 Chromosome Locus At5g66055   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 16 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Uncertain Function Details Ankyrin repeat protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Syngenta (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2109 nucleotides mRNA: 1607 nucleotides Protein:   435 amino acids cDNA Status FLcDNA Available: AY052363 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (83%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 247079_at

Mutant Information Allele Symbol emb 2036-1 emb 2036-2 emb 16 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta D. Meinke Mutant Line Number 54841 78350 123B Ecotype Columbia Columbia Columbia Genetic Background   qrt   ABRC Stock CS16041 CS16042   Mutagen Treatment T-DNA T-DNA EMS Insertion Mutant Yes   Details Yes   Details No Flanking Sequence Details  (Help)     Location of Mutation 5' UTR to Exon 1     Terminal Phenotype Globular  Details Globular  Details Globular Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test Complementation Test Database Release March, 2002 March, 2002 December, 2010 Seed Color White White   Embryo Color White White   Percent Mutant Seeds 24.7 % 23.8 % 24.5 % Chi - Square 0.3 0.4   Seeds Counted 935 628   Percent Top Half 53.9 % 49.3 % 47.8 % Chi - Square 1.5 <0.1   Average Seed Length 480 ± 40 µm 520 ± 30 µm   Average Embryo Length 80 ± 20 µm 60 ± 10 µm