OSU Syngenta vbi

SeedGenes Profile: EMB 2219

Gene Information
Gene Symbol EMB 2219
Chromosome Locus At2g21710   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details mTERF domain protein
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:2533 nucleotides
mRNA: 2034 nucleotides
Protein:  640 amino acids
cDNA Status FLcDNA Available: AY219048
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (60%)
Model Genomes:   None
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 263899_at
    
Mutant Information
Allele Symbol emb 2219-1emb 2219-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Salk
Mutant Line Number 71079SALK_100040
Ecotype ColumbiaColumbia
Genetic Background qrt 
ABRC Stock CS16044CS24081
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help) 
Location of Mutation Exon 1Intron 4
Terminal Phenotype Transition  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002March, 2004
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 24.3 %26.0 %
Chi - Square 0.20.3
Seeds Counted 1048562
Percent Top Half 50.2 %49.0 %
Chi - Square <0.10.1
Average Seed Length 480 ± 50 µm570 ± 60 µm
Average Embryo Length 120 ± 50 µm230 ± 80 µm