SeedGenes Profile: EMB 2279

Gene Information Gene Symbol EMB 2279 Chromosome Locus At1g30610   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 88 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function PPR Protein Function Details Processing of organellar mRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Vernon / Salk (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 3842 nucleotides mRNA: 3021 nucleotides Protein:   1006 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT5G67570.1 (40%) Plant ESTs: Nicotiana tabacum (56%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 263220_at

Mutant Information Allele Symbol emb 2279-1 emb 2279-2 emb 2279-3 emb 88 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Vernon / Salk Vernon / Salk Meinke / DuPont Mutant Line Number 75549 SALK_088420 SALK_086107 1002 Ecotype Columbia Columbia Columbia Wassilewskija Genetic Background qrt       ABRC Stock CS16174       Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)       Location of Mutation Exon 2 to Intron 2 Exon 4 Exon 7   Terminal Phenotype Globular  Details Not Available Not Available Globular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test Database Release March, 2003 December, 2010 December, 2010 December, 2010 Seed Color White       Embryo Color White       Percent Mutant Seeds 27.6 %     27.7 % Chi - Square 3.2       Seeds Counted 895       Percent Top Half 54.3 %     48.4 % Chi - Square 1.8       Average Seed Length 500 ± 50 µm       Average Embryo Length 80 ± 20 µm