OSU Syngenta vbi

SeedGenes Profile: EMB 2279

Gene Information
Gene Symbol EMB 2279
Chromosome Locus At1g30610   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol EMB 88 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function PPR Protein
Function Details Processing of organellar mRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Vernon / Salk (2 alleles)
Database Release March, 2003
Predicted Sequences
Gene:3842 nucleotides
mRNA: 3021 nucleotides
Protein:  1006 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: AT5G67570.1 (40%)
Plant ESTs: Nicotiana tabacum (56%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 263220_at
    
Mutant Information
Allele Symbol emb 2279-1emb 2279-2emb 2279-3emb 88
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaVernon / SalkVernon / SalkMeinke / DuPont
Mutant Line Number 75549SALK_088420SALK_0861071002
Ecotype ColumbiaColumbiaColumbiaWassilewskija
Genetic Background qrt   
ABRC Stock CS16174   
Mutagen Treatment T-DNAT-DNAT-DNAT-DNA
Insertion Mutant Yes   DetailsYes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)   
Location of Mutation Exon 2 to Intron 2Exon 4Exon 7 
Terminal Phenotype Globular  DetailsNot AvailableNot AvailableGlobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence IdentityComplementation Test
Database Release March, 2003December, 2010December, 2010December, 2010
Seed Color White   
Embryo Color White   
Percent Mutant Seeds 27.6 %  27.7 %
Chi - Square 3.2   
Seeds Counted 895   
Percent Top Half 54.3 %  48.4 %
Chi - Square 1.8   
Average Seed Length 500 ± 50 µm   
Average Embryo Length 80 ± 20 µm