SeedGenes Profile: EMB 2423

Gene Information Gene Symbol EMB 2423 Chromosome Locus At3g48470   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Weak similarity to telomere maintenance protein (Telo2) Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 5124 nucleotides mRNA: 3112 nucleotides Protein:   1017 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (43%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA

Mutant Information Allele Symbol emb 2423-1 emb 2423-2 emb 2423-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Salk Mutant Line Number 87045 80223 SALK_097304 Ecotype Columbia Columbia Columbia Genetic Background   qrt   ABRC Stock CS16215 CS16216 CS24086 Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Exon 1   Exon 1 Terminal Phenotype Preglobular  Details Cotyledon  Details Transition  Details Nomarski Phenotype Globular  Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2003 March, 2003 March, 2004 Seed Color White White to Pale Yellow White Embryo Color White White to Pale Yellow White Percent Mutant Seeds 20.8 % 25.0 % 25.9 % Chi - Square 8.8 <0.1 0.4 Seeds Counted 946 1158 1008 Percent Top Half 53.8 % 55.0 % 52.9 % Chi - Square 1.1 2.9 0.8 Average Seed Length 510 ± 40 µm 500 ± 40 µm 480 ± 70 µm Average Embryo Length 15 ± 0 µm 170 ± 90 µm 120 ± 50 µm