SeedGenes Profile: EMB 2453

Gene Information Gene Symbol EMB 2453 Chromosome Locus At4g39620   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function PPR Protein Function Details Processing of organellar mRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Vernon / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2285 nucleotides mRNA: 1692 nucleotides Protein:   563 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Spruce (57%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 252904_at

Mutant Information Allele Symbol emb 2453-1 emb 2453-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Vernon / Salk Mutant Line Number 77105 SALK_071838 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock CS16054   Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 1 Promoter, 3' UTR, or Exon 1 Terminal Phenotype Globular  Details Cotyledon Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 December, 2010 Seed Color White   Embryo Color White   Percent Mutant Seeds 27.7 %   Chi - Square 3.6   Seeds Counted 947   Percent Top Half 49.2 %   Chi - Square <0.1   Average Seed Length 480 ± 60 µm   Average Embryo Length 90 ± 50 µm