SeedGenes Profile: EMB 2726

Gene Information Gene Symbol EMB 2726 Chromosome Locus At4g29060   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Translation Elongation Factor Ts Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Beligni / SAIL (2 alleles) Database Release September, 2002 Predicted Sequences Gene: 4312 nucleotides mRNA: 3348 nucleotides Protein:   953 amino acids cDNA Status FLcDNA Available: AY056792 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (80%) Model Genomes:   Synechocystis (64%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 253758_at

Mutant Information Allele Symbol emb 2726-1 emb 2726-2 emb 2726-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Beligni / SAIL Beligni / SAIL Mutant Line Number 129824 SAIL_1252_B12 SAIL_540_B10 Ecotype Columbia Columbia Columbia Genetic Background       ABRC Stock CS16123     Mutagen Treatment T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)     Location of Mutation Exon 7 to Downstream Intron 5 Exon 6 Terminal Phenotype Globular  Details Not Available Not Available Nomarski Phenotype   Images Allelism Evidence Reference Allele Sequence Identity Sequence Identity Database Release September, 2002 October, 2007 October, 2007 Seed Color White     Embryo Color White     Percent Mutant Seeds 24.3 %     Chi - Square 0.3     Seeds Counted 1028     Percent Top Half 46.8 %     Chi - Square 1.0     Average Seed Length 490 ± 50 µm     Average Embryo Length 70 ± 20 µm