SeedGenes Profile: EMB 2737

Gene Information Gene Symbol EMB 2737 Chromosome Locus At5g53860   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol EMB 64 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Unknown Function Details Putative auxin-regulated protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2003 Predicted Sequences Gene: 3575 nucleotides mRNA: 1620 nucleotides Protein:   422 amino acids cDNA Status FLcDNA Available: AY050429 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Cotton (Gossypium) (79%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 248235_at

Mutant Information Allele Symbol emb 2737-1 emb 2737-2 emb 64 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Ecker Mutant Line Number 116042 SALK_083393 L3-3H Ecotype Columbia Columbia Columbia Genetic Background qrt     ABRC Stock CS16187 CS24089   Mutagen Treatment T-DNA T-DNA Irradiation Insertion Mutant Yes   Details Yes   Details No Flanking Sequence Details  (Help)     Location of Mutation Intron 9 Intron 3   Terminal Phenotype Cotyledon  Details Cotyledon  Details Cotyledon Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test Database Release March, 2003 March, 2004 December, 2010 Seed Color White White   Embryo Color White White   Percent Mutant Seeds 26.6 % 24.7 % 23.9 % Chi - Square 1.6 0.1   Seeds Counted 1182 1006   Percent Top Half 52.5 % 52.0 % 50.3 % Chi - Square 0.8 0.4   Average Seed Length 550 ± 40 µm 530 ± 60 µm   Average Embryo Length 440 ± 60 µm 150 ± 80 µm