SeedGenes Profile: EMB 2750

Gene Information Gene Symbol EMB 2750 Chromosome Locus At3g06430   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function PPR Protein Function Details Processing of organellar mRNAs Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Myouga / Riken (3 alleles) Database Release September, 2003 Predicted Sequences Gene: 1891 nucleotides mRNA: 1769 nucleotides Protein:   486 amino acids cDNA Status FLcDNA Available: AY099616 Top BLAST Hits Arabidopsis: AT3G53170.1 (40%) Plant ESTs: Brassica napus (88%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 2750-1 emb 2750-2 emb 2750-3 emb 2750-4 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Myouga / Riken Myouga / Riken Myouga / Riken Mutant Line Number 66280 RATM13-4177-1 RATM15-2099-1 RATM15-2352-1 Ecotype Columbia Nossen Nossen Nossen Genetic Background qrt       ABRC Stock CS16224       Mutagen Treatment T-DNA Transposon Transposon Transposon Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)       Location of Mutation Exon 2       Terminal Phenotype Cotyledon  Details Globular Globular Globular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release September, 2003 December, 2010 December, 2010 December, 2010 Seed Color White to Pale Yellow       Embryo Color White to Pale Yellow       Percent Mutant Seeds 24.6 % 27.2 % 23.0 % 29.3 % Chi - Square 0.1 0.5 0.6 2.5 Seeds Counted 1038 250 239 256 Percent Top Half 47.8 %       Chi - Square 0.5       Average Seed Length 510 ± 70 µm       Average Embryo Length 310 ± 80 µm