OSU Syngenta vbi

SeedGenes Profile: EMB 2750

Gene Information
Gene Symbol EMB 2750
Chromosome Locus At3g06430   tair
Alleles in Database 4   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function PPR Protein
Function Details Processing of organellar mRNAs
Gene Evidence
Double Border Recovery
Meinke / Syngenta (1 allele)
Border Recovery
Myouga / Riken (3 alleles)
Database Release September, 2003
Predicted Sequences
Gene:1891 nucleotides
mRNA: 1769 nucleotides
Protein:  486 amino acids
cDNA Status FLcDNA Available: AY099616
Top BLAST Hits
Arabidopsis: AT3G53170.1 (40%)
Plant ESTs: Brassica napus (88%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
    
Mutant Information
Allele Symbol emb 2750-1emb 2750-2emb 2750-3emb 2750-4
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMyouga / RikenMyouga / RikenMyouga / Riken
Mutant Line Number 66280RATM13-4177-1RATM15-2099-1RATM15-2352-1
Ecotype ColumbiaNossenNossenNossen
Genetic Background qrt   
ABRC Stock CS16224   
Mutagen Treatment T-DNATransposonTransposonTransposon
Insertion Mutant Yes   DetailsYes   DetailsYes   DetailsYes   Details
Flanking Sequence Details  (Help)   
Location of Mutation Exon 2   
Terminal Phenotype Cotyledon  DetailsGlobularGlobularGlobular
Nomarski Phenotype
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentityComplementation Test; Sequence IdentityComplementation Test; Sequence Identity
Database Release September, 2003December, 2010December, 2010December, 2010
Seed Color White to Pale Yellow   
Embryo Color White to Pale Yellow   
Percent Mutant Seeds 24.6 %27.2 %23.0 %29.3 %
Chi - Square 0.10.50.62.5
Seeds Counted 1038250239256
Percent Top Half 47.8 %   
Chi - Square 0.5   
Average Seed Length 510 ± 70 µm   
Average Embryo Length 310 ± 80 µm