SeedGenes Profile: EMB 2757

Gene Information Gene Symbol EMB 2757 Chromosome Locus At4g29860   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol TAN (J. Harada) Sequence Identity Gene Class Embryo Defective Predicted Function Uncertain Function Details WD-40 repeat protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Syngenta (1 allele) Molecular Complementation J. Harada (1 allele) Border Recovery J. Harada (1 allele) Border Recovery Harada / Salk (1 allele) Database Release September, 2004 Predicted Sequences Gene: 4099 nucleotides mRNA: 2011 nucleotides Protein:   386 amino acids cDNA Status FLcDNA Available: BX826964 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (84%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA

Mutant Information Allele Symbol emb 2757-1 emb 2757-2 tan-1 tan-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Harada / Feldmann Harada / Salk Mutant Line Number 75602 46177 24 SALK_097510 Ecotype Columbia Columbia Wassilewskija Columbia Genetic Background qrt       ABRC Stock         Mutagen Treatment T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help)       Location of Mutation Intron 7 to Exon 8   Intron 12 Exon 9 Terminal Phenotype Cotyledon  Details Cotyledon  Details Cotyledon Cotyledon Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Sequence Identity Database Release September, 2004 September, 2004 October, 2007 October, 2007 Seed Color Pale Yellow Pale Yellow     Embryo Color Pale Yellow Pale Yellow     Percent Mutant Seeds 26.2 % 26.2 %     Chi - Square 0.7 0.8     Seeds Counted 901 1112     Percent Top Half 53.4 % 49.5 %     Chi - Square 1.1 <0.1     Average Seed Length 540 ± 70 µm 580 ± 70 µm     Average Embryo Length 470 ± 80 µm 450 ± 80 µm