SeedGenes Profile: EMB 2773

Gene Information Gene Symbol EMB 2773 Chromosome Locus At5g15540   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol AtSCC 2 (I. Siddiqi) Sequence Identity Gene Class Embryo Defective Predicted Function Adherin (AtSCC2) Function Details Sister chromatid cohesion Gene Evidence Single Border Recovery Meinke / Salk (2 alleles) Database Release October, 2007 Predicted Sequences Gene: 9739 nucleotides mRNA: 5482 nucleotides Protein:   1755 amino acids cDNA Status FLcDNA Available: EF520004 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (54%) Model Genomes:   Homo sapiens (26%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 246558_at

Mutant Information Allele Symbol emb 2773-1 emb 2773-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_151609 SALK_052585 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 9 Exon 24 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Preglobular  Images Early Globular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release October, 2007 October, 2007 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.6 % 25.6 % Chi - Square 0.1 0.2 Seeds Counted 1112 931 Percent Top Half 53.8 % 50.8 % Chi - Square 1.6 0.1 Average Seed Length 470 ± 100 µm 510 ± 70 µm Average Embryo Length 20 ± 40 µm 15 ± 0 µm