OSU Syngenta vbi

SeedGenes Profile: EMB 2776

Gene Information
Gene Symbol EMB 2776
Chromosome Locus At2g41500   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol LIS (U. Grossniklaus) Sequence Identity
PRP 4 (S. Hoth) Sequence Identity
Gene Class Embryo Defective
Predicted Function U4/U6 snRNP (Prp4)
Function Details Proposed role in pre-mRNA splicing
Gene Evidence
Molecular Complementation
S. Hoth (1 allele)
Border Recovery
Meinke; Grossniklaus; Hoth / Sal (1 allele)
Sequenced Mutant Allele
U. Grossniklaus (1 allele)
Molecular Complementation
U. Grossniklaus (1 allele)
Border Recovery
Hoth / WiscDsLox (1 allele)
Database Release October, 2007
Predicted Sequences
Gene:3297 nucleotides
mRNA: 2060 nucleotides
Protein:  554 amino acids
cDNA Status FLcDNA Available: AK229530
Top BLAST Hits
Arabidopsis: AT2G05720.1 (50%)
Plant ESTs: Vitis vinifera (69%)
Model Genomes:  Danio rerio (44%)
Subcellular Localization TargetP Targeted to Chloroplasts
mRNA Expression Data NASCArray Spot History for 267102_at
Mutant Information
Allele Symbol emb 2776lis-1prp 4-2
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke; Hoth; Grossniklaus / SaU. GrossniklausHoth / WiscDsLox
Mutant Line Number SALK_070009 WISC476B11
Ecotype ColumbiaLandsberg erecta 
Genetic Background    
ABRC Stock    
Mutagen Treatment T-DNAEMSTransposon
Insertion Mutant Yes   DetailsNoYes   Details
Flanking Sequence    
Location of Mutation Intron 2Exon 3Exon 4
Terminal Phenotype Preglobular  DetailsPreglobularPreglobular
Nomarski Phenotype
Allelism Evidence Reference AlleleSequence IdentitySequence Identity
Database Release October, 2007October, 2007October, 2007
Seed Color White  
Embryo Color White  
Percent Mutant Seeds 14.2 %  
Chi - Square 53.2  
Seeds Counted 863  
Percent Top Half 39.8 %  
Chi - Square 5.1  
Average Seed Length 460 ± 110 µm  
Average Embryo Length 30 ± 30 µm