SeedGenes Profile: EMB 2776

Gene Information Gene Symbol EMB 2776 Chromosome Locus At2g41500   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol LIS (U. Grossniklaus) Sequence Identity PRP 4 (S. Hoth) Sequence Identity Gene Class Embryo Defective Predicted Function U4/U6 snRNP (Prp4) Function Details Proposed role in pre-mRNA splicing Gene Evidence Molecular Complementation S. Hoth (1 allele) Border Recovery Meinke; Grossniklaus; Hoth / Sal (1 allele) Sequenced Mutant Allele U. Grossniklaus (1 allele) Molecular Complementation U. Grossniklaus (1 allele) Border Recovery Hoth / WiscDsLox (1 allele) Database Release October, 2007 Predicted Sequences Gene: 3297 nucleotides mRNA: 2060 nucleotides Protein:   554 amino acids cDNA Status FLcDNA Available: AK229530 Top BLAST Hits Arabidopsis: AT2G05720.1 (50%) Plant ESTs: Vitis vinifera (69%) Model Genomes:   Danio rerio (44%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 267102_at

Mutant Information Allele Symbol emb 2776 lis-1 prp 4-2 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke; Hoth; Grossniklaus / Sa U. Grossniklaus Hoth / WiscDsLox Mutant Line Number SALK_070009   WISC476B11 Ecotype Columbia Landsberg erecta   Genetic Background       ABRC Stock       Mutagen Treatment T-DNA EMS Transposon Insertion Mutant Yes   Details No Yes   Details Flanking Sequence       Location of Mutation Intron 2 Exon 3 Exon 4 Terminal Phenotype Preglobular  Details Preglobular Preglobular Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Sequence Identity Database Release October, 2007 October, 2007 October, 2007 Seed Color White     Embryo Color White     Percent Mutant Seeds 14.2 %     Chi - Square 53.2     Seeds Counted 863     Percent Top Half 39.8 %     Chi - Square 5.1     Average Seed Length 460 ± 110 µm     Average Embryo Length 30 ± 30 µm