SeedGenes Profile: EMB 2789

Gene Information Gene Symbol EMB 2789 Chromosome Locus At5g05680   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol MOS 7 (X. Li) Sequence Identity Gene Class Embryo Defective Predicted Function Nuclear Envelope Protein (Nup88) Function Details Nuclear protein export Gene Evidence Border Recovery Meinke / Salk (2 alleles) Database Release December, 2010 Predicted Sequences Gene: 4376 nucleotides mRNA: 2698 nucleotides Protein:   810 amino acids cDNA Status FLcDNA Available: AK229039 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (87%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 250769_at

Mutant Information Allele Symbol emb 2789-1 emb 2789-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Salk Meinke / Salk Mutant Line Number SALK_085349 SALK_129301 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 6 Intron 3 Terminal Phenotype Preglobular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color White to Pale Yellow White to Pale Yellow Embryo Color White to Pale Yellow White to Pale Yellow Percent Mutant Seeds 28.3 % 23.1 % Chi - Square 1.5 0.5 Seeds Counted 254 251 Percent Top Half 47.2 % 51.7 % Chi - Square 0.2 0.1 Average Seed Length 490 ± 40 µm 510 ± 30 µm Average Embryo Length 20 ± 20 µm 30 ± 30 µm