OSU Syngenta vbi

SeedGenes Profile: EMB 30

Gene Information
Gene Symbol EMB 30
Chromosome Locus At1g13980   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol GN (G. Jurgens) Sequence Identity
Gene Class Embryo Defective
Predicted Function ARF Guanine Exchange Factor
Function Details Large membrane-associated ARF GEF; role in vesicle trafficking and cell polarity
Gene Evidence
Sequenced Mutant Allele
D. Shevell (1 allele)
Border Recovery
D. Shevell (1 allele)
Sequenced Mutant Allele
G. Jurgens (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:6096 nucleotides
mRNA: 4835 nucleotides
Protein:  1451 amino acids
cDNA Status FLcDNA Available: U36432
Top BLAST Hits
Arabidopsis: AT5G39500.1 (62%)
Plant ESTs: Vitis vinifera (83%)
Model Genomes:  Caenorhabditis elegans (31%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 262614_at
    
Mutant Information
Allele Symbol emb 30-1gnomemb 30-3
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant D. MeinkeG. JurgensShevell / Feldmann
Mutant Line Number 112A-2AT391 
Ecotype ColumbiaLandsberg erectaWassilewskija
Genetic Background    
ABRC Stock CS6320 CS6322
Mutagen Treatment EMSEMST-DNA
Insertion Mutant NoNoYes   Details
Flanking Sequence    
Location of Mutation Exon 3Exon 3Exon 2
Terminal Phenotype Cotyledon  DetailsCotyledon  DetailsCotyledon
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentitySequence Identity
Database Release March, 2002March, 2002March, 2002
Seed Color GreenGreen 
Embryo Color GreenGreen 
Percent Mutant Seeds 23.4 %25.5 % 
Chi - Square 0.6<0.1 
Seeds Counted 4741040 
Percent Top Half 55.9 %49.8 % 
Chi - Square 1.5<0.1 
Average Seed Length 510 ± 30 µm430 ± 40 µm 
Average Embryo Length 340 ± 70 µm280 ± 90 µm