SeedGenes Profile: EMB 30

Gene Information Gene Symbol EMB 30 Chromosome Locus At1g13980   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol GN (G. Jurgens) Sequence Identity Gene Class Embryo Defective Predicted Function ARF Guanine Exchange Factor Function Details Large membrane-associated ARF GEF; role in vesicle trafficking and cell polarity Gene Evidence Sequenced Mutant Allele D. Shevell (1 allele) Border Recovery D. Shevell (1 allele) Sequenced Mutant Allele G. Jurgens (1 allele) Database Release March, 2002 Predicted Sequences Gene: 6096 nucleotides mRNA: 4835 nucleotides Protein:   1451 amino acids cDNA Status FLcDNA Available: U36432 Top BLAST Hits Arabidopsis: AT5G39500.1 (62%) Plant ESTs: Vitis vinifera (83%) Model Genomes:   Caenorhabditis elegans (31%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 262614_at

Mutant Information Allele Symbol emb 30-1 gnom emb 30-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant D. Meinke G. Jurgens Shevell / Feldmann Mutant Line Number 112A-2A T391   Ecotype Columbia Landsberg erecta Wassilewskija Genetic Background       ABRC Stock CS6320   CS6322 Mutagen Treatment EMS EMS T-DNA Insertion Mutant No No Yes   Details Flanking Sequence       Location of Mutation Exon 3 Exon 3 Exon 2 Terminal Phenotype Cotyledon  Details Cotyledon  Details Cotyledon Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Database Release March, 2002 March, 2002 March, 2002 Seed Color Green Green   Embryo Color Green Green   Percent Mutant Seeds 23.4 % 25.5 %   Chi - Square 0.6 <0.1   Seeds Counted 474 1040   Percent Top Half 55.9 % 49.8 %   Chi - Square 1.5 <0.1   Average Seed Length 510 ± 30 µm 430 ± 40 µm   Average Embryo Length 340 ± 70 µm 280 ± 90 µm