SeedGenes Profile: EMB 3123

Gene Information Gene Symbol EMB 3123 Chromosome Locus At3g27750   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details VPS9 domain protein Gene Evidence Border Recovery Myouga / Riken (1 allele) Border Recovery Meinke / Salk (1 allele) Database Release December, 2010 Predicted Sequences Gene: 1067 nucleotides mRNA: 1067 nucleotides Protein:   222 amino acids cDNA Status FLcDNA Available: AF375402 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (71%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 258232_at

Mutant Information Allele Symbol emb 3123-1 emb 3123-2 Mutant Class Embryo Defective Seed Pigment Source of Mutant Myouga / Riken Meinke / Salk Mutant Line Number RATM12-5139-1 SALK_140334 Ecotype Nossen Columbia Genetic Background     ABRC Stock     Mutagen Treatment Transposon T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 1 Exon 1 Terminal Phenotype Cotyledon Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 26.0 % 21.2 % Chi - Square 0.5 2.4 Seeds Counted 864 321 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length