OSU Syngenta vbi

SeedGenes Profile: GLA 1

Gene Information
Gene Symbol GLA 1
Chromosome Locus At5g41480   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol EMB 9 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function Dihydrofolate Synthetase
Function Details Folate biosynthesis
Gene Evidence
Molecular Complementation
Y. Machida (1 allele)
Border Recovery
Y. Machida (1 allele)
Database Release March, 2003
Predicted Sequences
Gene:2710 nucleotides
mRNA: 1746 nucleotides
Protein:  530 amino acids
cDNA Status FLcDNA Available: BX831692
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (87%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
mRNA Expression Data NASCArray Spot History for 249304_at
Mutant Information
Allele Symbol gla 1-1gla 1-1Semb 9
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Y. MachidaMeinke / SyngentaD. Meinke
Mutant Line Number 754528879A
Ecotype Landsberg erectaColumbiaColumbia
Genetic Background    
ABRC Stock  CS24060 
Mutagen Treatment T-DNAT-DNAEMS
Insertion Mutant Yes   DetailsYes   DetailsNo
Flanking Sequence  Details  (Help) 
Location of Mutation Intron 4 to Exon 5Exon 8 to Intron 8 
Terminal Phenotype GlobularPreglobular  DetailsPreglobular
Nomarski Phenotype   ImagesGlobular
Allelism Evidence Reference AlleleSequence IdentityComplementation Test
Database Release March, 2003March, 2004December, 2010
Seed Color  White 
Embryo Color  White 
Percent Mutant Seeds  26.3 %23.9 %
Chi - Square  1.0 
Seeds Counted  948 
Percent Top Half  55.2 %59.9 %
Chi - Square  2.7 
Average Seed Length  430 ± 60 µm 
Average Embryo Length  15 ± 0 µm