SeedGenes Profile: PDE 327

Gene Information Gene Symbol PDE 327 Chromosome Locus At4g30720   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Seed Pigment Predicted Function Unknown Function Details None Gene Evidence Border Recovery Myouga / Salk (1 allele) Border Recovery Myouga / SAIL (1 allele) Database Release December, 2010 Predicted Sequences Gene: 3614 nucleotides mRNA: 2488 nucleotides Protein:   707 amino acids cDNA Status FLcDNA Available: AK226777 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (67%) Model Genomes:   Synechocystis (42%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 253585_at

Mutant Information Allele Symbol pde 327-1 pde 327-2 Mutant Class Seed Pigment Seed Pigment Source of Mutant Myouga / Riken Myouga / Riken Mutant Line Number SALK_059716 SAIL_20_E03 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Exon 3 Exon 3 Terminal Phenotype Pigment Defective Pigment Defective Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Database Release December, 2010 December, 2010 Seed Color     Embryo Color     Percent Mutant Seeds 25.2 % 19.8 % Chi - Square <0.1 3.7 Seeds Counted 591 243 Percent Top Half     Chi - Square     Average Seed Length     Average Embryo Length