SeedGenes Profile: SMT 1

Gene Information Gene Symbol SMT 1 Chromosome Locus At5g13710   Alleles in Database 4   See List OR Show All Identity Confidence Confirmed Alias Symbol CPH (G. Jurgens) Sequence Identity Gene Class Embryo Defective Predicted Function Sterol Methyltransferase (Yeast Erg6p) Function Details Sterol biosynthesis; modulation of cholesterol levels Gene Evidence Sequenced Mutant Allele G. Jurgens (1 allele) Double Border Recovery G. Fink (2 alleles) Phenotypic Reversion G. Fink (1 allele) Sequenced Mutant Allele G. Fink (1 allele) Database Release September, 2002 Predicted Sequences Gene: 3364 nucleotides mRNA: 1473 nucleotides Protein:   336 amino acids cDNA Status FLcDNA Available: AF195648 Top BLAST Hits Arabidopsis: AT1G76090.1 (41%) Plant ESTs: Brassica napus (95%) Model Genomes:   Saccharomyces cerevisiae (53%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 250254_at

Mutant Information Allele Symbol smt 1-1 smt 1-2 smt 1-3 cph-T357 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant G. Fink G. Fink G. Fink G. Jurgens Mutant Line Number         Ecotype Wassilewskija Wassilewskija Wassilewskija Landsberg erecta Genetic Background         ABRC Stock         Mutagen Treatment Transposon Transposon Transposon EMS Insertion Mutant Yes   Details Yes   Details No No Flanking Sequence         Location of Mutation Intron 2 Exon 12 Exon 10 Sterol-Binding Pocket Terminal Phenotype Cotyledon Cotyledon Cotyledon Cotyledon Nomarski Phenotype Allelism Evidence Reference Allele Sequence Identity Sequence Identity Sequence Identity Database Release September, 2002 September, 2002 September, 2002 September, 2002 Seed Color         Embryo Color         Percent Mutant Seeds         Chi - Square         Seeds Counted         Percent Top Half         Chi - Square         Average Seed Length         Average Embryo Length