OSU Syngenta vbi

SeedGenes Profile: BIO 1

Gene Information
Gene Symbol BIO 1
Chromosome Locus At5g57590   tair
Alleles in Database 6   See List OR Show All
Identity Confidence Confirmed
Alias Symbol BIO 3 (D. Meinke) Alternate Name
Gene Class Embryo Defective
Predicted Function 7-KAP, DAP Aminotransferase; Dethiobiotin Synthetase
Function Details Biotin synthesis in mitochondria; Bifunctional (BIO3-BIO1) protein
Gene Evidence
Experimental Data
D. Meinke (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Multigene Disruption
Meinke / Syngenta (1 allele)
Border Recovery
Meinke / Riken (2 alleles)
Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:4263 nucleotides
mRNA: 2670 nucleotides
Protein:  833 amino acids
cDNA Status FLcDNA Available: EU089963
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (58%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
mRNA Expression Data NASCArray Spot History for 247916_at
Mutant Information
Allele Symbol bio 3-1
Mutant Class Embryo Defective
Source of Mutant Meinke / Riken
Mutant Line Number RATM53-2665-1
Ecotype Nossen
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence  
Location of Mutation Exon 1
Terminal Phenotype Cotyledon  Details
Nomarski Phenotype  
Allelism Evidence Complementation Test; Sequence Identity
Database Release October, 2007
Seed Color Pale Yellow-Green
Embryo Color Pale Yellow-Green
Percent Mutant Seeds 23.5 %
Chi - Square 1.5
Seeds Counted 1210
Percent Top Half 50.7 %
Chi - Square 0.1
Average Seed Length 510 ± 30 µm
Average Embryo Length 210 ± 80 µm