SeedGenes Profile: BIO 1

Gene Information Gene Symbol BIO 1 Chromosome Locus At5g57590   Alleles in Database 6   See List OR Show All Identity Confidence Confirmed Alias Symbol BIO 3 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function 7-KAP, DAP Aminotransferase; Dethiobiotin Synthetase Function Details Biotin synthesis in mitochondria; Bifunctional (BIO3-BIO1) protein Gene Evidence Experimental Data D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Multigene Disruption Meinke / Syngenta (1 allele) Border Recovery Meinke / Riken (2 alleles) Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4263 nucleotides mRNA: 2670 nucleotides Protein:   833 amino acids cDNA Status FLcDNA Available: EU089963 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 247916_at

Mutant Information Allele Symbol bio 3-1 Mutant Class Embryo Defective Source of Mutant Meinke / Riken Mutant Line Number RATM53-2665-1 Ecotype Nossen Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 1 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release October, 2007 Seed Color Pale Yellow-Green Embryo Color Pale Yellow-Green Percent Mutant Seeds 23.5 % Chi - Square 1.5 Seeds Counted 1210 Percent Top Half 50.7 % Chi - Square 0.1 Average Seed Length 510 ± 30 µm Average Embryo Length 210 ± 80 µm