SeedGenes Profile: BIO 1

Gene Information Gene Symbol BIO 1 Chromosome Locus At5g57590   Alleles in Database 6   See List OR Show All Identity Confidence Confirmed Alias Symbol BIO 3 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function 7-KAP, DAP Aminotransferase; Dethiobiotin Synthetase Function Details Biotin synthesis in mitochondria; Bifunctional (BIO3-BIO1) protein Gene Evidence Experimental Data D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Multigene Disruption Meinke / Syngenta (1 allele) Border Recovery Meinke / Riken (2 alleles) Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4263 nucleotides mRNA: 2670 nucleotides Protein:   833 amino acids cDNA Status FLcDNA Available: EU089963 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (58%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 247916_at

Mutant Information Allele Symbol bio 1-2 bio 1-3 bio 1-1 bio 3-1 bio 3-2 bio 3-3 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta D. Meinke Meinke / Riken Meinke / Riken Meinke / Salk Mutant Line Number 36172 46455 122G-E RATM53-2665-1 RATM53-3000-1 SALK_023399 Ecotype Columbia Columbia Columbia Nossen Nossen Columbia Genetic Background             ABRC Stock CS16001 CS16002 CS6316       Mutagen Treatment T-DNA T-DNA EMS T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details No Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details         Location of Mutation Exon 8 Downstream to At5g57580 Intron 13 Exon 1 Exon 1 Exon 2 Terminal Phenotype Cotyledon  Details Cotyledon  Details Cotyledon  Details Cotyledon  Details Cotyledon  Details Cotyledon  Details Nomarski Phenotype   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Database Release March, 2002 March, 2002 March, 2002 October, 2007 October, 2007 October, 2007 Seed Color Pale Yellow White Pale Yellow-Green Pale Yellow-Green Pale Yellow-Green Pale Yellow Embryo Color Pale Yellow White Pale Yellow-Green Pale Yellow-Green Pale Yellow-Green Pale Yellow Percent Mutant Seeds 27.0 % 34.8 % 23.1 % 23.5 % 23.8 % 24.3 % Chi - Square 3.3 51.0 2.2 1.5 1.0 0.3 Seeds Counted 1045 994 1156 1210 1233 1182 Percent Top Half 48.9 % 55.8 % 48.3 % 50.7 % 53.6 % 48.1 % Chi - Square 0.1 2.3 0.3 0.1 1.5 0.4 Average Seed Length 510 ± 40 µm 510 ± 40 µm 510 ± 40 µm 510 ± 30 µm 510 ± 20 µm 540 ± 50 µm Average Embryo Length 300 ± 100 µm 320 ± 90 µm 270 ± 80 µm 210 ± 80 µm 190 ± 50 µm 230 ± 110 µm