SeedGenes Profile: EMB 1067

Gene Information Gene Symbol EMB 1067 Chromosome Locus At2g45330   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol TRPT 1 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function 2' tRNA Phosphotransferase Function Details Catalytic role in tRNA splicing Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Riken (1 allele) Database Release March, 2002 Predicted Sequences Gene: 1700 nucleotides mRNA: 1035 nucleotides Protein:   289 amino acids cDNA Status FLcDNA Available: AK228262 Top BLAST Hits Arabidopsis: AT5G23600.1 (95%) Plant ESTs: Brassica napus (93%) Model Genomes:   Danio rerio (41%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 245131_s_at

Mutant Information Allele Symbol emb 1067-1 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 7724 Ecotype Columbia Genetic Background   ABRC Stock CS16010 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 6 Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Allelism Evidence Reference Allele Database Release March, 2002 Seed Color Pale Yellow-Green Embryo Color Pale Yellow-Green Percent Mutant Seeds 23.4 % Chi - Square 1.3 Seeds Counted 1016 Percent Top Half 50.4 % Chi - Square <0.1 Average Seed Length 490 ± 60 µm Average Embryo Length 290 ± 80 µm