SeedGenes Profile: EMB 1067

Gene Information Gene Symbol EMB 1067 Chromosome Locus At2g45330   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol TRPT 1 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function 2' tRNA Phosphotransferase Function Details Catalytic role in tRNA splicing Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Meinke / Riken (1 allele) Database Release March, 2002 Predicted Sequences Gene: 1700 nucleotides mRNA: 1035 nucleotides Protein:   289 amino acids cDNA Status FLcDNA Available: AK228262 Top BLAST Hits Arabidopsis: AT5G23600.1 (95%) Plant ESTs: Brassica napus (93%) Model Genomes:   Danio rerio (41%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 245131_s_at

Mutant Information Allele Symbol emb 1067-1 emb 1067-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Riken Mutant Line Number 7724 RATM13-1397-1 Ecotype Columbia Nossen Genetic Background     ABRC Stock CS16010   Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 6   Terminal Phenotype Cotyledon  Details Globular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 December, 2010 Seed Color Pale Yellow-Green   Embryo Color Pale Yellow-Green   Percent Mutant Seeds 23.4 % 26.0 % Chi - Square 1.3 0.2 Seeds Counted 1016 570 Percent Top Half 50.4 %   Chi - Square <0.1   Average Seed Length 490 ± 60 µm   Average Embryo Length 290 ± 80 µm