SeedGenes Profile: EMB 1273

Gene Information Gene Symbol EMB 1273 Chromosome Locus At1g49510   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details None Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 1722 nucleotides mRNA: 1196 nucleotides Protein:   240 amino acids cDNA Status FLcDNA Available: AK227372 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (78%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 262394_at

Mutant Information Allele Symbol emb 1273-1 emb 1273-2 Mutant Class Embryo Defective Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 16479 SALK_004621 Ecotype Uncertain Columbia Genetic Background     ABRC Stock CS16016 CS24063 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Intron 1 to Exon 3 Exon 1 Terminal Phenotype Cotyledon  Details Pigment Defective  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 26.9 % 25.4 % Chi - Square 2.0 0.1 Seeds Counted 1010 913 Percent Top Half 47.8 % 48.7 % Chi - Square 0.5 0.1 Average Seed Length 480 ± 40 µm 550 ± 60 µm Average Embryo Length 330 ± 50 µm 460 ± 50 µm