OSU Syngenta vbi

SeedGenes Profile: EMB 1275

Gene Information
Gene Symbol EMB 1275
Chromosome Locus At1g55350   tair
Alleles in Database 6   See List OR Show All
Identity Confidence Confirmed
Alias Symbol AtDEK 1 (Olsen; Ingram) Sequence Identity
EMB 80 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function Calpain-Type Cysteine Protease
Function Details Similar to maize DEK1
Gene Evidence
Border Recovery
Olsen / Dellaporta (1 allele)
Border Recovery
Olsen / GABI (1 allele)
Border Recovery
Ingram / Salk (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Syngenta (1 allele)
Database Release September, 2004
Predicted Sequences
Gene:10803 nucleotides
mRNA: 7220 nucleotides
Protein:  2151 amino acids
cDNA Status FLcDNA Available: AY061803
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (70%)
Model Genomes:  Danio rerio (34%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 259646_at
Mutant Information
Allele Symbol emb 1275-1
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 16696
Ecotype Columbia
Genetic Background  
ABRC Stock  
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence Details  (Help)
Location of Mutation Intron 5 to Exon 6
Terminal Phenotype Preglobular  Details
Nomarski Phenotype   Images
Allelism Evidence Reference Allele
Database Release September, 2004
Seed Color White
Embryo Color White
Percent Mutant Seeds 21.8 %
Chi - Square 5.6
Seeds Counted 1010
Percent Top Half 53.6 %
Chi - Square 1.2
Average Seed Length 460 ± 70 µm
Average Embryo Length 15 ± 0 µm