OSU Syngenta vbi

SeedGenes Profile: EMB 1275

Gene Information
Gene Symbol EMB 1275
Chromosome Locus At1g55350   tair
Alleles in Database 6   See List OR Show All
Identity Confidence Confirmed
Alias Symbol AtDEK 1 (Olsen; Ingram) Sequence Identity
EMB 80 (D. Meinke) Complemetation Test
Gene Class Embryo Defective
Predicted Function Calpain-Type Cysteine Protease
Function Details Similar to maize DEK1
Gene Evidence
Border Recovery
Olsen / Dellaporta (1 allele)
Border Recovery
Olsen / GABI (1 allele)
Border Recovery
Ingram / Salk (1 allele)
Double Border Recovery
Meinke / Syngenta (1 allele)
Single Border Recovery
Meinke / Syngenta (1 allele)
Database Release September, 2004
Predicted Sequences
Gene:10803 nucleotides
mRNA: 7220 nucleotides
Protein:  2151 amino acids
cDNA Status FLcDNA Available: AY061803
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Rice (Oryza sativa) (70%)
Model Genomes:  Danio rerio (34%)
Subcellular Localization TargetP Not Targeted to Cell Compartment
mRNA Expression Data NASCArray Spot History for 259646_at
Mutant Information
Allele Symbol emb 1275-1emb 1275-2dek 1-1dek 1-2dek 1-3emb 80
Mutant Class Embryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / SyngentaOlsen / DellaportaOlsen / GABIIngram / SAILMeinke / DuPont
Mutant Line Number 16696102227 GABI_228D09SAIL_384_F0798
Ecotype ColumbiaColumbiaColumbiaColumbiaColumbiaWassilewskija
Genetic Background       
ABRC Stock       
Insertion Mutant Yes   DetailsYes   DetailsYes   DetailsYes   DetailsYes   DetailsNo
Flanking Sequence Details  (Help)Details    
Location of Mutation Intron 5 to Exon 6Exon 29Intron 11Exon 3Intron 21 
Terminal Phenotype Preglobular  DetailsGlobular  DetailsGlobularNot AvailableGlobularPreglobular
Nomarski Phenotype   Images  ImagesGlobular
Allelism Evidence Reference AlleleComplementation Test; Sequence IdentitySequence IdentitySequence IdentitySequence IdentityComplementation Test
Database Release September, 2004September, 2004October, 2007October, 2007October, 2007December, 2010
Seed Color WhiteWhite    
Embryo Color WhiteWhite    
Percent Mutant Seeds 21.8 %35.5 %   23.4 %
Chi - Square 5.654.8    
Seeds Counted 1010926    
Percent Top Half 53.6 %50.2 %   50.4 %
Chi - Square 1.2<0.1    
Average Seed Length 460 ± 70 µm530 ± 70 µm    
Average Embryo Length 15 ± 0 µm60 ± 30 µm