SeedGenes Profile: EMB 1275

Gene Information Gene Symbol EMB 1275 Chromosome Locus At1g55350   Alleles in Database 6   See List OR Show All Identity Confidence Confirmed Alias Symbol AtDEK 1 (Olsen; Ingram) Sequence Identity EMB 80 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Calpain-Type Cysteine Protease Function Details Similar to maize DEK1 Gene Evidence Border Recovery Olsen / Dellaporta (1 allele) Border Recovery Olsen / GABI (1 allele) Border Recovery Ingram / Salk (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2004 Predicted Sequences Gene: 10803 nucleotides mRNA: 7220 nucleotides Protein:   2151 amino acids cDNA Status FLcDNA Available: AY061803 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (70%) Model Genomes:   Danio rerio (34%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 259646_at

Mutant Information Allele Symbol emb 1275-1 emb 1275-2 dek 1-1 dek 1-2 dek 1-3 emb 80 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Olsen / Dellaporta Olsen / GABI Ingram / SAIL Meinke / DuPont Mutant Line Number 16696 102227   GABI_228D09 SAIL_384_F07 98 Ecotype Columbia Columbia Columbia Columbia Columbia Wassilewskija Genetic Background             ABRC Stock             Mutagen Treatment T-DNA T-DNA T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details No Flanking Sequence Details  (Help) Details         Location of Mutation Intron 5 to Exon 6 Exon 29 Intron 11 Exon 3 Intron 21   Terminal Phenotype Preglobular  Details Globular  Details Globular Not Available Globular Preglobular Nomarski Phenotype   Images   Images Globular Allelism Evidence Reference Allele Complementation Test; Sequence Identity Sequence Identity Sequence Identity Sequence Identity Complementation Test Database Release September, 2004 September, 2004 October, 2007 October, 2007 October, 2007 December, 2010 Seed Color White White         Embryo Color White White         Percent Mutant Seeds 21.8 % 35.5 %       23.4 % Chi - Square 5.6 54.8         Seeds Counted 1010 926         Percent Top Half 53.6 % 50.2 %       50.4 % Chi - Square 1.2 <0.1         Average Seed Length 460 ± 70 µm 530 ± 70 µm         Average Embryo Length 15 ± 0 µm 60 ± 30 µm