OSU Syngenta vbi

SeedGenes Profile: EMB 1579

Gene Information
Gene Symbol EMB 1579
Chromosome Locus At2g03150   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Uncertain
Function Details Putative calcium binding protein
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Single Border Recovery
Meinke / Salk (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:7233 nucleotides
mRNA: 4375 nucleotides
Protein:  1340 amino acids
cDNA Status FLcDNA Available: AK228824
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Soybean (Glycine max) (61%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Mitochondria
SUBA
mRNA Expression Data NASCArray Spot History for 266727_at
    
Mutant Information
Allele Symbol emb 1579-1emb 1579-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 2964957679
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16026CS16027
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help)Details
Location of Mutation Intron 18Exon 6 to Exon 12
Terminal Phenotype Cotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2002March, 2002
Seed Color WhiteWhite to Pale Yellow
Embryo Color WhiteWhite to Pale Yellow
Percent Mutant Seeds 21.9 %25.3 %
Chi - Square 4.9<0.1
Seeds Counted 9631115
Percent Top Half 55.5 %56.0 %
Chi - Square 2.52.1
Average Seed Length 440 ± 60 µm450 ± 30 µm
Average Embryo Length 120 ± 110 µm240 ± 120 µm