SeedGenes Profile: EMB 1586

Gene Information Gene Symbol EMB 1586 Chromosome Locus At1g12770   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol ISE 1 (D. Meinke) Sequence Identity Gene Class Embryo Defective Predicted Function DEAD/DEAH Box RNA Helicase Function Details RNA binding protein; potential target of gene silencing; putative role in trafficking of clathrin-coated vesicles Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Sequenced Mutant Allele P. Zambryski (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2622 nucleotides mRNA: 2069 nucleotides Protein:   551 amino acids cDNA Status FLcDNA Available: AK175587 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (63%) Model Genomes:   Synechocystis (29%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 261194_at

Mutant Information Allele Symbol emb 1586-1 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 8388 Ecotype Columbia Genetic Background   ABRC Stock CS16028 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Upstream Terminal Phenotype Cotyledon  Details Nomarski Phenotype   Images Allelism Evidence Reference Allele Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 27.4 % Chi - Square 3.3 Seeds Counted 1115 Percent Top Half 48.2 % Chi - Square 0.4 Average Seed Length 530 ± 40 µm Average Embryo Length 250 ± 120 µm