SeedGenes Profile: EMB 1586

Gene Information Gene Symbol EMB 1586 Chromosome Locus At1g12770   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol ISE 1 (D. Meinke) Sequence Identity Gene Class Embryo Defective Predicted Function DEAD/DEAH Box RNA Helicase Function Details RNA binding protein; potential target of gene silencing; putative role in trafficking of clathrin-coated vesicles Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Sequenced Mutant Allele P. Zambryski (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2622 nucleotides mRNA: 2069 nucleotides Protein:   551 amino acids cDNA Status FLcDNA Available: AK175587 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (63%) Model Genomes:   Synechocystis (29%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 261194_at

Mutant Information Allele Symbol emb 1586-1 emb 1586-2 ise 1-1 Mutant Class Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta P. Zambryski Mutant Line Number 8388 29863   Ecotype Columbia Columbia Landsberg erecta Genetic Background       ABRC Stock CS16028 CS16029   Mutagen Treatment T-DNA T-DNA EMS Insertion Mutant Yes   Details Yes   Details No Flanking Sequence Details  (Help) Details   Location of Mutation Upstream Exon 1 Exon 1 Terminal Phenotype Cotyledon  Details Globular  Details Cotyledon Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test Database Release March, 2002 March, 2002 September, 2004 Seed Color White White   Embryo Color White White   Percent Mutant Seeds 27.4 % 24.9 %   Chi - Square 3.3 <0.1   Seeds Counted 1115 899   Percent Top Half 48.2 % 50.9 %   Chi - Square 0.4 <0.1   Average Seed Length 530 ± 40 µm 480 ± 50 µm   Average Embryo Length 250 ± 120 µm 70 ± 30 µm