SeedGenes Profile: EMB 1586

Gene Information Gene Symbol EMB 1586 Chromosome Locus At1g12770   Alleles in Database 3   See List OR Show All Identity Confidence Confirmed Alias Symbol ISE 1 (D. Meinke) Sequence Identity Gene Class Embryo Defective Predicted Function DEAD/DEAH Box RNA Helicase Function Details RNA binding protein; potential target of gene silencing; putative role in trafficking of clathrin-coated vesicles Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Sequenced Mutant Allele P. Zambryski (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2622 nucleotides mRNA: 2069 nucleotides Protein:   551 amino acids cDNA Status FLcDNA Available: AK175587 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (63%) Model Genomes:   Synechocystis (29%) Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 261194_at

Mutant Information Allele Symbol emb 1586-2 Mutant Class Embryo Defective Source of Mutant Meinke / Syngenta Mutant Line Number 29863 Ecotype Columbia Genetic Background   ABRC Stock CS16029 Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence Details  (Help) Location of Mutation Exon 1 Terminal Phenotype Globular  Details Nomarski Phenotype   Images Allelism Evidence Complementation Test; Sequence Identity Database Release March, 2002 Seed Color White Embryo Color White Percent Mutant Seeds 24.9 % Chi - Square <0.1 Seeds Counted 899 Percent Top Half 50.9 % Chi - Square <0.1 Average Seed Length 480 ± 50 µm Average Embryo Length 70 ± 30 µm