OSU Syngenta vbi

SeedGenes Profile: EMB 1586

Gene Information
Gene Symbol EMB 1586
Chromosome Locus At1g12770   tair
Alleles in Database 3   See List OR Show All
Identity Confidence Confirmed
Alias Symbol ISE 1 (D. Meinke) Sequence Identity
Gene Class Embryo Defective
Predicted Function DEAD/DEAH Box RNA Helicase
Function Details RNA binding protein; potential target of gene silencing; putative role in trafficking of clathrin-coated vesicles
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Sequenced Mutant Allele
P. Zambryski (1 allele)
Database Release March, 2002
Predicted Sequences
Gene:2622 nucleotides
mRNA: 2069 nucleotides
Protein:  551 amino acids
cDNA Status FLcDNA Available: AK175587
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (63%)
Model Genomes:  Synechocystis (29%)
Subcellular Localization TargetP Targeted to Mitochondria
mRNA Expression Data NASCArray Spot History for 261194_at
Mutant Information
Allele Symbol emb 1586-2
Mutant Class Embryo Defective
Source of Mutant Meinke / Syngenta
Mutant Line Number 29863
Ecotype Columbia
Genetic Background  
ABRC Stock CS16029
Mutagen Treatment T-DNA
Insertion Mutant Yes   Details
Flanking Sequence Details  (Help)
Location of Mutation Exon 1
Terminal Phenotype Globular  Details
Nomarski Phenotype   Images
Allelism Evidence Complementation Test; Sequence Identity
Database Release March, 2002
Seed Color White
Embryo Color White
Percent Mutant Seeds 24.9 %
Chi - Square <0.1
Seeds Counted 899
Percent Top Half 50.9 %
Chi - Square <0.1
Average Seed Length 480 ± 50 µm
Average Embryo Length 70 ± 30 µm