SeedGenes Profile: EMB 1865

Gene Information Gene Symbol EMB 1865 Chromosome Locus At3g18390   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Chloroplast Splicing Factor (CFM1) Function Details Splicing of group II introns in chloroplasts Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Border Recovery Myouga / Riken (1 allele) Database Release March, 2002 Predicted Sequences Gene: 4487 nucleotides mRNA: 3021 nucleotides Protein:   848 amino acids cDNA Status FLcDNA Available: AY063908 Top BLAST Hits Arabidopsis: AT3G01370.1 (43%) Plant ESTs: Vitis vinifera (66%) Model Genomes:   None Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 257717_at

Mutant Information Allele Symbol emb 1865-1 emb 1865-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Myouga / Riken Mutant Line Number 42644 RATM16-1117-1 Ecotype Columbia Nossen Genetic Background     ABRC Stock CS16036   Mutagen Treatment T-DNA Transposon Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 2   Terminal Phenotype Globular  Details Globular Nomarski Phenotype Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 December, 2010 Seed Color White   Embryo Color White   Percent Mutant Seeds 30.3 % 24.7 % Chi - Square 9.1 <0.1 Seeds Counted 604 279 Percent Top Half 48.1 %   Chi - Square 0.3   Average Seed Length 510 ± 50 µm   Average Embryo Length 70 ± 30 µm