OSU Syngenta vbi

SeedGenes Profile: EMB 1923

Gene Information
Gene Symbol EMB 1923
Chromosome Locus At4g28210   tair
Alleles in Database 2   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Unknown
Function Details None
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Database Release March, 2003
Predicted Sequences
Gene:1462 nucleotides
mRNA: 1211 nucleotides
Protein:  348 amino acids
cDNA Status FLcDNA Available: AK226695
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Brassica napus (82%)
Model Genomes:   None
Subcellular Localization TargetP Targeted to Chloroplasts
SUBA
mRNA Expression Data NASCArray Spot History for 253816_at
    
Mutant Information
Allele Symbol emb 1923-1emb 1923-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 13303839002
Ecotype ColumbiaColumbia
Genetic Background   
ABRC Stock CS16160CS16159
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help)Details
Location of Mutation Exon 25Õ UTR to Exon 1
Terminal Phenotype Cotyledon  DetailsCotyledon  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release March, 2003March, 2003
Seed Color WhiteWhite
Embryo Color WhiteWhite
Percent Mutant Seeds 27.4 %25.0 %
Chi - Square 4.1<0.1
Seeds Counted 13221034
Percent Top Half 48.4 %52.1 %
Chi - Square 0.30.2
Average Seed Length 510 ± 30 µm500 ± 50 µm
Average Embryo Length 160 ± 60 µm190 ± 90 µm