SeedGenes Profile: EMB 2001

Gene Information Gene Symbol EMB 2001 Chromosome Locus At2g22870   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details Putative ribosome biogenesis GTPase Gene Evidence Border Recovery Meinke / Syngenta (1 allele) Double Border Recovery Meinke / Syngenta (1 allele) Database Release September, 2004 Predicted Sequences Gene: 1648 nucleotides mRNA: 1105 nucleotides Protein:   300 amino acids cDNA Status FLcDNA Available: AY087277 Top BLAST Hits Arabidopsis: AT5G11480.1 (54%) Plant ESTs: Brassica napus (85%) Model Genomes:   None Subcellular Localization TargetP Targeted to Mitochondria SUBA mRNA Expression Data NASCArray Spot History for 266801_at

Mutant Information Allele Symbol emb 2001-1 emb 2001-2 Mutant Class Embryo Defective Seed Pigment Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 54846 98507 Ecotype Columbia Columbia Genetic Background     ABRC Stock     Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence   Details  (Help) Location of Mutation   Exon 1 Terminal Phenotype Transition  Details Cotyledon Nomarski Phenotype   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2004 September, 2004 Seed Color White Pale Yellow Embryo Color White Pale Yellow Percent Mutant Seeds 25.3 % 24.9 % Chi - Square 0.1 <0.1 Seeds Counted 1105 936 Percent Top Half 51.8 % 48.5 % Chi - Square 0.4 0.2 Average Seed Length 510 ± 60 µm   Average Embryo Length 100 ± 40 µm