OSU Syngenta vbi

SeedGenes Profile: EMB 2016

Gene Information
Gene Symbol EMB 2016
Chromosome Locus At3g05680   tair
Alleles in Database 5   See List OR Show All
Identity Confidence Confirmed
Alias Symbol  
Gene Class Embryo Defective
Predicted Function Unknown
Function Details None
Gene Evidence
Double Border Recovery
Meinke / Syngenta (2 alleles)
Single Border Recovery
Meinke / Syngenta (2 alleles)
Database Release September, 2003
Predicted Sequences
Gene:10495 nucleotides
mRNA: 6424 nucleotides
Protein:  2057 amino acids
cDNA Status EST Identified
Top BLAST Hits
Arabidopsis: Only self hit
Plant ESTs: Vitis vinifera (46%)
Model Genomes:   None
Subcellular Localization TargetP Not Targeted to Cell Compartment
SUBA
mRNA Expression Data NASCArray Spot History for 258891_at
    
Mutant Information
Allele Symbol emb 2016-1emb 2016-2
Mutant Class Embryo DefectiveEmbryo Defective
Source of Mutant Meinke / SyngentaMeinke / Syngenta
Mutant Line Number 51106114438
Ecotype ColumbiaColumbia
Genetic Background  qrt
ABRC Stock CS16203CS16204
Mutagen Treatment T-DNAT-DNA
Insertion Mutant Yes   DetailsYes   Details
Flanking Sequence Details  (Help)Details
Location of Mutation UpstreamExon 22
Terminal Phenotype Globular  DetailsGlobular  Details
Nomarski Phenotype   Images  Images
Allelism Evidence Reference AlleleComplementation Test; Sequence Identity
Database Release September, 2003September, 2003
Seed Color Pale YellowPale Yellow
Embryo Color Pale YellowPale Yellow
Percent Mutant Seeds 25.5 %21.4 %
Chi - Square 0.96.5
Seeds Counted 647970
Percent Top Half 50.9 %52.4 %
Chi - Square <0.10.5
Average Seed Length 540 ± 60 µm530 ± 60 µm
Average Embryo Length 80 ± 30 µm90 ± 40 µm