SeedGenes Profile: EMB 2016

Gene Information Gene Symbol EMB 2016 Chromosome Locus At3g05680   Alleles in Database 5   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Unknown Function Details None Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Single Border Recovery Meinke / Syngenta (2 alleles) Database Release September, 2003 Predicted Sequences Gene: 10495 nucleotides mRNA: 6424 nucleotides Protein:   2057 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Vitis vinifera (46%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 258891_at

Mutant Information Allele Symbol emb 2016-1 emb 2016-2 emb 2016-3 emb 2016-4 emb 2016-5 Mutant Class Embryo Defective Embryo Defective Embryo Defective Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Meinke / Syngenta Mutant Line Number 51106 114438 123805 36492 11879 Ecotype Columbia Columbia Columbia Columbia Columbia Genetic Background   qrt       ABRC Stock CS16203 CS16204 CS16205 CS16206 CS16207 Mutagen Treatment T-DNA T-DNA T-DNA T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Yes   Details Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Details Details   Location of Mutation Upstream Exon 22 Intron 2 Intron 15 to Exon 16   Terminal Phenotype Globular  Details Globular  Details Transition  Details Globular  Details Globular  Details Nomarski Phenotype   Images   Images   Images   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test; Sequence Identity Complementation Test Database Release September, 2003 September, 2003 September, 2003 September, 2003 September, 2003 Seed Color Pale Yellow Pale Yellow Pale Green Pale Yellow Pale Yellow Embryo Color Pale Yellow Pale Yellow Pale Green Pale Yellow Pale Yellow Percent Mutant Seeds 25.5 % 21.4 % 27.1 % 20.9 % 27.3 % Chi - Square 0.9 6.5 2.2 10.0 2.6 Seeds Counted 647 970 934 1111 954 Percent Top Half 50.9 % 52.4 % 51.0 % 64.2 % 47.6 % Chi - Square <0.1 0.5 0.1 18.8 0.6 Average Seed Length 540 ± 60 µm 530 ± 60 µm 510 ± 60 µm 500 ± 60 µm 430 ± 60 µm Average Embryo Length 80 ± 30 µm 90 ± 40 µm 110 ± 50 µm 70 ± 30 µm 70 ± 40 µm