SeedGenes Profile: EMB 2191

Gene Information Gene Symbol EMB 2191 Chromosome Locus At1g48175   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol TAD 2 (D. Meinke) Alternate Name Gene Class Embryo Defective Predicted Function tRNA Adenosine Deaminase Function Details Catalytic (TAD2) subunit; Modification of wobble position Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release March, 2003 Predicted Sequences Gene: 1825 nucleotides mRNA: 890 nucleotides Protein:   182 amino acids cDNA Status FLcDNA Available: BT014757 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Brassica napus (87%) Model Genomes:   Homo sapiens (50%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA

Mutant Information Allele Symbol emb 2191-1 emb 2191-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 68880 92954 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock CS16170 CS16171 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence     Location of Mutation Intron 4 Intron 1 to Intron 2 Terminal Phenotype Preglobular  Details Preglobular  Details Nomarski Phenotype Early Globular  Images Early Globular  Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2003 March, 2003 Seed Color White White Embryo Color White White Percent Mutant Seeds 23.2 % 24.2 % Chi - Square 1.4 0.4 Seeds Counted 794 1221 Percent Top Half 54.9 % 49.5 % Chi - Square 2.0 <0.1 Average Seed Length 370 ± 60 µm 400 ± 70 µm Average Embryo Length 15 ± 0 µm 15 ± 0 µm