SeedGenes Profile: EMB 2219

Gene Information Gene Symbol EMB 2219 Chromosome Locus At2g21710   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Uncertain Function Details mTERF domain protein Gene Evidence Double Border Recovery Meinke / Syngenta (1 allele) Single Border Recovery Meinke / Salk (1 allele) Database Release March, 2002 Predicted Sequences Gene: 2533 nucleotides mRNA: 2034 nucleotides Protein:   640 amino acids cDNA Status FLcDNA Available: AY219048 Top BLAST Hits Arabidopsis: Only self hit Plant ESTs: Rice (Oryza sativa) (60%) Model Genomes:   None Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 263899_at

Mutant Information Allele Symbol emb 2219-1 emb 2219-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Salk Mutant Line Number 71079 SALK_100040 Ecotype Columbia Columbia Genetic Background qrt   ABRC Stock CS16044 CS24081 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help)   Location of Mutation Exon 1 Intron 4 Terminal Phenotype Transition  Details Cotyledon  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release March, 2002 March, 2004 Seed Color White White Embryo Color White White Percent Mutant Seeds 24.3 % 26.0 % Chi - Square 0.2 0.3 Seeds Counted 1048 562 Percent Top Half 50.2 % 49.0 % Chi - Square <0.1 0.1 Average Seed Length 480 ± 50 µm 570 ± 60 µm Average Embryo Length 120 ± 50 µm 230 ± 80 µm