SeedGenes Profile: EMB 2247

Gene Information Gene Symbol EMB 2247 Chromosome Locus At5g16715   Alleles in Database 2   See List OR Show All Identity Confidence Confirmed Alias Symbol   Gene Class Embryo Defective Predicted Function Valine tRNA Synthetase Function Details Translation in chloroplast Gene Evidence Double Border Recovery Meinke / Syngenta (2 alleles) Database Release September, 2003 Predicted Sequences Gene: 8168 nucleotides mRNA: 3204 nucleotides Protein:   970 amino acids cDNA Status FLcDNA Available: BX830792 Top BLAST Hits Arabidopsis: AT1G14610.1 (39%) Plant ESTs: Rice (Oryza sativa) (77%) Model Genomes:   Synechocystis (43%) Subcellular Localization TargetP Targeted to Chloroplasts SUBA mRNA Expression Data NASCArray Spot History for 246509_at

Mutant Information Allele Symbol emb 2247-1 emb 2247-2 Mutant Class Embryo Defective Embryo Defective Source of Mutant Meinke / Syngenta Meinke / Syngenta Mutant Line Number 73553 67816 Ecotype Columbia Columbia Genetic Background qrt qrt ABRC Stock CS16212 CS16213 Mutagen Treatment T-DNA T-DNA Insertion Mutant Yes   Details Yes   Details Flanking Sequence Details  (Help) Details Location of Mutation Intron 18 to Exon 19 Exon 4 to Intron 4 Terminal Phenotype Globular  Details Globular  Details Nomarski Phenotype   Images   Images Allelism Evidence Reference Allele Complementation Test; Sequence Identity Database Release September, 2003 September, 2003 Seed Color White White Embryo Color White White Percent Mutant Seeds 25.3 % 27.7 % Chi - Square <0.1 4.1 Seeds Counted 945 1084 Percent Top Half 48.5 % 51.7 % Chi - Square 0.2 0.3 Average Seed Length 520 ± 70 µm 520 ± 60 µm Average Embryo Length 80 ± 30 µm 70 ± 30 µm