SeedGenes Profile: EMB 2284

Gene Information Gene Symbol EMB 2284 Chromosome Locus At1g08260   Alleles in Database 7   See List OR Show All Identity Confidence Confirmed Alias Symbol TIL 1 (P. Jenik) Sequence Identity EMB 529 (M. Devic) Sequence Identity TIL 1 (P. Jenik) Complemetation Test EMB 142 (D. Meinke) Complemetation Test Gene Class Embryo Defective Predicted Function Subunit of DNA Polymerase Epsilon Function Details DNA replication and perhaps DNA repair and cell cycle control Gene Evidence Border Recovery D. Meinke (1 allele) Double Border Recovery Meinke / Syngenta (3 alleles) Border Recovery Devic / Salk (1 allele) Border Recovery Devic / Versailles (1 allele) Sequenced Mutant Allele P. Jenik (1 allele) Database Release March, 2002 Predicted Sequences Gene: 15949 nucleotides mRNA: 6816 nucleotides Protein:   2271 amino acids cDNA Status EST Identified Top BLAST Hits Arabidopsis: AT2G27120.1 (79%) Plant ESTs: Rice (Oryza sativa) (57%) Model Genomes:   Homo sapiens (44%) Subcellular Localization TargetP Not Targeted to Cell Compartment SUBA mRNA Expression Data NASCArray Spot History for 261811_at

Mutant Information Allele Symbol emb 142 Mutant Class Embryo Defective Source of Mutant Meinke / DuPont Mutant Line Number 2977 Ecotype Wassilewskija Genetic Background   ABRC Stock   Mutagen Treatment T-DNA Insertion Mutant Yes   Details Flanking Sequence   Location of Mutation Exon 3 Terminal Phenotype Globular Nomarski Phenotype   Allelism Evidence Complementation Test; Sequence Identity Database Release December, 2010 Seed Color   Embryo Color   Percent Mutant Seeds 27.3 % Chi - Square   Seeds Counted   Percent Top Half 47.3 % Chi - Square   Average Seed Length   Average Embryo Length